All rights reserved. Lingqian Chang (Last updated June. 27, 2017)
(1) BioMEMS Devices: gene delivery, transfection, detection, regenerative medicine
Silicon Micro-/nano- Fabrication: 3D nano-channel array chip based on silicon nanofabrication (E-beam lithography, projection photolithography, DRIE (Bosch Process), PECVD, silicon wet etch) for manipulation of million cells on a single chip. 3D Nano-electroporation Technique: For the first time, a 3D nano-electroporation system (3D NEP) for high throughput cells transfections with high-efficiency, cell safety and dose control, moving towards the clinically valuable Bio-chips. Intracellular Cancer Cell Sensor: Delivered intracellular probe (molecular beacons) into patient cancer cells for interrogation of stem-like cells and characterized their properties and drug resistance at single cell level. Cell Transfection Chip: For the first time, the 3D NEP successfully transfected the mammalian cardiomyocytes in vitro with dose control and high safety. High-throughput Gene chip: Multiplexed single-cell testing drug resistance of stem cells using designed intracellular mRNA markers. Revealed the heterogeneity in cancer stem cells strongly related to the drug resistance and metastasis in tumor environments. In Vivo Patchable Gene Delivery Chip: NEP for in vivo direct reprogramming epithelial cells into functional neurons and endothelial cells for regenerative medicine.
(2) Lab-on-chip Systems
On-Chip Cell manipulation: Study of on-chip high-throughput cell manipulation based on advanced techniques, including (i) Magnetic tweezers; (ii) Dielectrophoresis (DEP); (iii) Thin-film Hydrodynamics; (iv) Optical tweezers. Microfluidics: Silicon / Polymer based microfluidic chips for cell manipulation, micro-/nano-particles synthesis and optical applications.
Electro-chemical Micro-sensors:Electro-chemical biosensor systems for applications in Blood glucose Micro-sensors for non-invasive measurement of Type-II diabetes Implantable Neuron Micro-sensors: Electrode Array for neurological detection. High-throughput Protein Sensors: Single protein detection based on living cell sampling and self-assembled membrane (SAM) for mass spectrometry detection. Environmental Nano-sensors: (i) Humidity nano-sensors for environmental monitoring; (iii) Polymeric sensors for pH, temperature, and ionic detection
(4) Polymer Micro-/nano-fabrication
Polymeric micro-/nano-fabrication: Nano-imprinting Lithography, Hot Embossing, Injection Molding, Soft-lithography. Synthetic nanoporous sensors: Polyelectrolyte/mesoporous silica hybrid materials for multiple detection of pH value, temperature and ion conductivity. Drug Delivery: Synthesized Dendrimers and PEG/heparin lipoplex nanoparticles for in vivo drug / gene delivery. Tissue engineering: Fabrication of bio-scaffold based on polymer materials for tissue engineering. Nanocomposites: Carbon nanotube/ polymer nanocomposite foams for electromagnetic interference shielding.
Teaching Assistant (Mentor), Introduction of Polymer Micro-/Nano-technologies (CHBE 799, Ohio State University), Graduate Course Clean-room techniques for micro-/nano-fabrication including E-beam lithography, photolithography, E-beam evaporation, deep reactive ion-etch.